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Firstly, regarding female participants, the menstrual cycle phase was not monitored by hormonal markers or ultrasonography throughout the study course. Still, none of studied women declared the presence of a menstrual cycle disorder that may have affected levels of circulating estrogen and therefore influenced the rate of fat oxidation. It would be valuable to include monitoring of the menstrual cycle in further investigations. Secondly, the lack of a control group may serve as a slight limitation of our study.
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